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A 1.5 Hour Procedure for Identification of Enterococcus Species Directly from Blood Cultures
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Published on: February 10, 2011

Modified colony indirect epifluorescence test for serotyping Ureaplasma urealyticum and an adaptation to detect

G W Stemke, J A Robertson

    Journal of Clinical Microbiology
    |November 1, 1981
    PubMed

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    Enzyme-Linked Immunosorbent Assay

    In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
    There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.