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Related Experiment Videos

Clindamycin uptake by human neutrophils.

M S Klempner, B Styrt

    The Journal of Infectious Diseases
    |November 1, 1981
    PubMed
    Summary

    Clindamycin is rapidly absorbed by human polymorphonuclear neutrophils (PMNs), enhancing their ability to kill microbes in anaerobic conditions. This uptake is pH-dependent, suggesting clindamycin is lysosomotropic.

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    Area of Science:

    • Pharmacology
    • Cell Biology
    • Immunology

    Background:

    • Anaerobic environments impair the antimicrobial functions of polymorphonuclear neutrophils (PMNs).
    • Enhancing PMN microbicidal capacity in low-oxygen conditions is crucial for treating certain infections.

    Purpose of the Study:

    • To investigate the uptake mechanisms of clindamycin by human PMNs.
    • To understand how clindamycin concentration and activity are affected within PMNs.

    Main Methods:

    • Studied clindamycin uptake kinetics in human PMNs under varying conditions.
    • Investigated the role of pH gradients and lysosomal accumulation.
    • Assessed the bioactivity of intracellular clindamycin.

    Main Results:

    • Clindamycin achieved intracellular concentrations 40 times higher than extracellular levels.
    • Uptake was rapid, saturable, temperature-dependent, and inhibited by acidic pH.
    • PMN lysosomes actively accumulated clindamycin, indicating a lysosomotropic mechanism.

    Conclusions:

    • Clindamycin is efficiently accumulated by human PMNs via a pH-gradient-dependent, lysosomotropic mechanism.
    • This enhanced intracellular concentration likely augments PMN microbicidal activity in anaerobic environments.
    • Clindamycin demonstrates significant potential for boosting neutrophil function during anaerobic infections.

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