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Histamine secretion from human leucocytes stimulated by basophil-derived platelet-activating factor
Scandinavian Journal of Immunology
|January 1, 1981
Summary
Platelet-activating factors (PAF) from basophils stimulate histamine release from leucocytes. This PAF component also augments histamine secretion during leucocyte activation, suggesting an endogenous feedback role.
Area of Science:
- Immunology
- Cell Biology
- Biochemistry
Background:
- Basophils are key immune cells involved in allergic responses.
- Platelet-activating factor (PAF) is a potent lipid mediator implicated in inflammation and platelet activation.
Purpose of the Study:
- To investigate the role of basophil-derived PAF in histamine secretion.
- To characterize the PAF component responsible for histamine release and its modulatory effects.
Main Methods:
- Methanolic extraction of PAF from antigen-challenged basophil supernatants.
- Preparative thin-layer chromatography for PAF component isolation.
- Co-incubation assays with human peripheral blood leucocytes to assess histamine release.
Main Results:
- Crude basophil extracts stimulated histamine secretion from leucocytes.
- A PAF component isolated via chromatography induced histamine release.
- This PAF component augmented histamine secretion induced by other stimuli (F-Met-Leu-Phe, antigen E).
Conclusions:
- Basophil-derived PAF acts as an endogenous feedback mechanism during basophil activation.
- PAF modulates platelet function and influences leucocyte histamine secretion.