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Related Experiment Videos

Dextran-sulfate: a mitogen for human T lymphocytes.

R Palacios, I Sugawara, C Fernandez

    Journal of Immunology (Baltimore, Md. : 1950)
    |February 1, 1982
    PubMed
    Summary

    Dextran-sulfate (DxS) stimulates human T lymphocyte proliferation, requiring accessory cells for activation and interleukin 2 production. This mitogenic effect on T cells is suppressed by Cyclosporin-A.

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    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Dextran-sulfate (DxS) is a polysaccharide with potential immunomodulatory properties.
    • Understanding lymphocyte activation pathways is crucial for immune system research.

    Purpose of the Study:

    • To investigate the mitogenic effects of Dextran-sulfate (DxS) on human T and B lymphocytes.
    • To elucidate the role of accessory cells and Cyclosporin-A in DxS-induced T cell responses.

    Main Methods:

    • Human peripheral blood lymphocytes (T and B cells) were cultured with DxS.
    • Accessory cells were either present or absent during stimulation.
    • Interleukin 2 production and DNA synthesis were measured.
    • The effect of Cyclosporin-A on T cell proliferation was assessed.

    Main Results:

    • DxS induced proliferation of human T lymphocytes but not adult or neonatal B lymphocytes.
    • Accessory cells were essential for DxS-induced T cell DNA synthesis and interleukin 2 production.
    • Cyclosporin-A significantly suppressed T cell proliferation by inhibiting interleukin 2 synthesis and response.

    Conclusions:

    • DxS acts as a mitogen for human T lymphocytes, but not for B lymphocytes.
    • Accessory cells play a critical role in mediating DxS-induced T cell activation.
    • Cyclosporin-A interferes with key pathways of T cell activation induced by DxS.

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