Effect of protein aggregation on murine splenic prostaglandin F2 alpha levels

Intravenous injection of deaggregated human gamma globulin and deaggregated keyhole limpet hemocyanin into C57Bl/6 mice does not result in an increase in splenic prostaglandin F2 alpha. However, when aggregated human gamma globulin or aggregated keyhole limpet hemocyanin were injected, significant increases in splenic PGF2 alpha were observed. Mice which had been rendered immunologically tolerant to human gamma globulin (HGG) also demonstrated increases in splenic PGF2 alpha and PGE2 5 min after boosting with aggregated HGG with no increases in antibody against HGG. These results imply that the property of protein aggregation will engender increases in splenic prostaglandin levels and that these increases don't appear to affect the tolerant state.