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Related Experiment Videos

Backscattered electron imaging of human leukocytes.

D Soligo, N Lampen, E de Harven

    Scanning Electron Microscopy
    |January 1, 1981
    PubMed
    Summary

    Backscattered electron imaging (BEI) effectively visualizes human leukocytes using elemental markers like iron and silver. These methods aid in identifying cell types and phagocytic activity in normal and malignant conditions.

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    Area of Science:

    • Hematology
    • Cell Biology
    • Microscopy

    Background:

    • Leukocyte characterization is crucial for diagnosing hematological disorders.
    • Advanced imaging techniques are needed for detailed cellular analysis.

    Purpose of the Study:

    • To evaluate backscattered electron imaging (BEI) for studying human leukocytes.
    • To explore the utility of elemental markers in BEI for cell identification.

    Main Methods:

    • Application of backscattered electron imaging (BEI).
    • Utilized elemental markers: iron carbonyl, silver staining, lead phosphate, and osmium/diaminobenzidine complex.
    • Investigated phagocytic activity, nuclear morphology, and enzyme localization.

    Main Results:

    • Iron carbonyl effectively marked phagocytic activity in granulocytes and monocytes.
    • Silver staining revealed nuclear shape and nucleo-cytoplasmic ratio, aiding polymorphonuclear cell identification.
    • Lead phosphate and osmium complexes identified specific leukemias and myeloid precursors, respectively.

    Conclusions:

    • BEI with elemental markers provides detailed insights into leukocyte morphology and function.
    • These techniques facilitate the identification of normal and malignant leukocytes.
    • Surface morphology is preserved, allowing correlation with internal markers.

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