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Strain-specific aggregation of enterovirus by dextran sulfate
Archives of Virology
|January 1, 1981
Summary
Dextran sulfate aggregates enteroviruses, impacting plaque formation and virion release. This effect depends on factors like pH, ionic strength, protein content, and virus strain.
Area of Science:
- Virology
- Biochemistry
Background:
- Enteroviruses are significant human pathogens.
- Dextran sulfate is known to inhibit viral plaque formation.
Purpose of the Study:
- To investigate the mechanism of dextran sulfate's inhibitory effect on enteroviruses.
- To determine the factors influencing dextran sulfate-induced enterovirus aggregation.
Main Methods:
- Filtration experiments
- Ultracentrifugation
- Electron microscopy
- Plaque assays
Main Results:
- Dextran sulfate induced enterovirus aggregation, influenced by pH, ionic strength, protein content, and viral strain.
- Aggregation correlated with inhibition of plaque formation and release of mature virions.
- Maximal effects on aggregation and viral release occurred at the same dextran sulfate concentration.
Conclusions:
- Dextran sulfate's inhibition of enterovirus plaque formation is primarily due to its virus-aggregating properties.
- The findings elucidate the antiviral mechanism of dextran sulfate against enteroviruses.