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Related Concept Videos

RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
RNA Structure01:19

RNA Structure

The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

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Related Experiment Video

Updated: Jul 16, 2026

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution
10:45

iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution

Published on: April 30, 2011

Correlation of hnRNP structure and nascent transcript cleavage.

A L Beyer, A H Bouton, O L Miller

    Cell
    |October 1, 1981
    PubMed
    Summary

    Specific cleavage of nascent transcripts in Drosophila and Calliphora involves RNP fibril loop formation. This process releases RNA fragments and occurs rapidly after transcription.

    Area of Science:

    • Molecular Biology
    • Cell Biology
    • Genetics

    Background:

    • Nascent RNA transcripts undergo complex processing.
    • Understanding RNA processing is crucial for gene expression regulation.

    Purpose of the Study:

    • To investigate the structural and temporal aspects of nascent transcript cleavage in Drosophila and Calliphora.
    • To characterize the morphology and secondary structure of cleaved RNA fragments.

    Main Methods:

    • Electron microscopy of spread chromatin.
    • Quantitative analysis of transcription units.
    • Observation of nascent RNA transcripts and their cleavage products.

    Main Results:

    • Observed specific cleavage of nascent transcripts in nonnucleolar transcription units.

    More Related Videos

    Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro
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    Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro

    Published on: May 3, 2014

    Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae
    09:12

    Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae

    Published on: February 27, 2026

    Related Experiment Videos

    Last Updated: Jul 16, 2026

    iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution
    10:45

    iCLIP - Transcriptome-wide Mapping of Protein-RNA Interactions with Individual Nucleotide Resolution

    Published on: April 30, 2011

    Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro
    09:16

    Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro

    Published on: May 3, 2014

    Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae
    09:12

    Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae

    Published on: February 27, 2026

  • Characterized RNP morphology: released fragments (50 A fibrillar), retained segments (250 A particulate).
  • Identified RNP fibril loop formation involving noncontiguous transcript sequences, preceding release of 1-25 kb segments.

    • Conclusions:

    • Nascent hnRNA transcript cleavage is a common event in these organisms.
    • Cleavage occurs rapidly (0.3-3 min) after transcription of the cleavage site.
    • Specific secondary structure formation (RNP loops) is integral to the cleavage mechanism.