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Related Experiment Videos

Ia.W39 expression correlates with a specific Ir function.

B T Huber, T H Hansen, R R Skelly

    Journal of Immunology (Baltimore, Md. : 1950)
    |May 1, 1982
    PubMed
    Summary
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    Mice with xid defects or bm12 mutations are unresponsive to beef insulin due to the absence of Ia.W39 specificity. This study reveals distinct genetic causes for the lack of Ia.W39 expression in these mouse models.

    Area of Science:

    • Immunology
    • Genetics
    • Molecular Biology

    Background:

    • Mice with H-2b haplotype normally respond to beef insulin.
    • Two mutant mouse strains, xid-defective and bm12, show unresponsiveness to beef insulin.
    • This unresponsiveness is linked to the absence of Ia.W39 specificity on M phi (macrophage) and B cell surfaces.

    Purpose of the Study:

    • To investigate the genetic basis of unresponsiveness to beef insulin in xid-defective and bm12 mutant mice.
    • To determine the role of Ia.W39 specificity in immune response to beef insulin.
    • To differentiate the molecular defects causing Ia.W39 absence in the two mutant strains.

    Main Methods:

    • Comparative analysis of mutant mouse strains (xid-defective and bm12).
    • Assessment of Ia.W39 specificity expression on M phi and B cell surfaces.

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  • Investigation of antigen synthesis and membrane expression.

    • Main Results:

    • Both xid-defective and bm12 mice lack Ia.W39 specificity, correlating with unresponsiveness to beef insulin.
    • Ia.W39 is identified as the specific Ir epitope for the beef insulin immune response.
    • Xid-defective mice fail to express Ia.W39 on the cell surface, likely due to a maturation defect, while bm12 mice cannot synthesize Ia.W39 due to a structural mutation.

    Conclusions:

    • The absence of Ia.W39 specificity is the direct cause of unresponsiveness to beef insulin in both mutant strains.
    • Xid represents a regulatory or maturation gene defect affecting Ia.W39 membrane expression.
    • Bm12 harbors a structural mutation in the Ia.W39 gene, impacting its synthesis.