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[Concentration of heterogeneous leukocyte interferon]
Voprosy Virusologii
|November 1, 1981
Summary
Ultrafiltration effectively concentrates porcine leukocyte interferon, enhancing its antiviral activity by removing inhibitors. This improved interferon shows promise for veterinary and medical applications.
Area of Science:
- Biotechnology
- Immunology
- Virology
Background:
- Porcine leukocyte interferon exhibits antiviral properties.
- Concentration methods are crucial for maximizing interferon efficacy.
- Existing methods may not fully exploit the therapeutic potential of interferons.
Purpose of the Study:
- To compare different concentration methods for porcine leukocyte interferon.
- To identify the most effective technique for concentrating interferon active in human cells.
- To evaluate the impact of concentration on interferon's antiviral activity and identify any associated inhibitory factors.
Main Methods:
- Comparative analysis of various concentration techniques.
- Application of ultrafiltration using a TCF-10 apparatus.
- Quantification of antiviral activity in IU/ml.
- Isolation and concentration of identified inhibitors.
Main Results:
- Ultrafiltration via TCF-10 proved to be the most effective concentration method.
- Concentrated porcine interferon achieved antiviral activity levels of up to 1 X 10(6) IU/ml.
- Total antiviral activity doubled post-concentration due to the removal of an antiviral inhibitor.
- The removed inhibitor was itself isolated and concentrated.
Conclusions:
- Ultrafiltration is a superior method for concentrating porcine leukocyte interferon.
- Concentration significantly enhances the antiviral potency of porcine interferon.
- The study identified and concentrated an inhibitor that reduced native interferon activity.
- Further trials in veterinary medicine and human medicine are warranted for the concentrated porcine interferon.