Histochemical modifications induced "in vitro" by electromagnetic stimulation of growing bone tissues

The histochemical modifications induced on a mouse limb bud culture system by electromagnetic stimulation have been investigated using mucopolysaccharide (MPS) staining methods. An increase in the amount of MPS with cartilage has been observed. This difference is more obvious on the more distal segments of the limb, i.e. on the youngest structures. These observations are correlated with some modifications observed in the enzymatic activities of acid phosphatase and of beta glucuronidase.