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The automated analytical electrophoresis microscope.

P H Bartels, G B Olson, H G Bartels

Cell Biophysics

|December 1, 1981

Summary

An automated analytical electrophoresis microscope (AEMS) enables rapid, reliable cell electrophoretic mobility measurements. This high-speed system collects statistically valid data for cell populations like lymphocytes and erythrocytes.

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Area of Science:

Biophysics
Cell Biology
Analytical Chemistry

Background:

Electrophoretic mobility is a key biophysical property of cells.
Accurate measurement of electrophoretic mobility is crucial for cell characterization.
Existing methods may be time-consuming or lack precision.

Purpose of the Study:

To describe the components and functionality of an automated computer-controlled analytical electrophoresis microscope (AEMS).
To demonstrate the system's capability for rapid and reliable electrophoretic mobility measurements.
To establish a foundation for high-speed data collection and analysis of cell populations.

Main Methods:

Development of an automated microscope system integrating computer control and video imaging.
Utilizing phase contrast microscopy and vidicon detection for cell projection.
Implementing computer algorithms for cell search, tracking, and velocity measurement.
Automated data acquisition for high-speed collection of multiple velocity data points per cell.

Main Results:

The automated computer-controlled analytical electrophoresis microscope (AEMS) system was successfully constructed and described.
The system enables rapid acquisition of multiple cell velocity measurements.
Reliable determinations of electrophoretic mobilities were achieved through computer-aided tracking.
Initial evaluations on lymphocytes, erythrocytes, and platelets demonstrated the system's utility.

Conclusions:

The AEMS provides a high-speed, automated solution for measuring cell electrophoretic mobility.
Computer-controlled algorithms facilitate rapid data collection, enabling statistically valid profiles.
The system is effective for analyzing electrophoretic properties of various cell types, including blood cells.

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