Related Experiment Video

Updated: Aug 8, 2026

Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
10:32

Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells

Published on: June 5, 2015

Cell-bound immunoglobulin on peripheral blood mononuclear cells of patients with myeloma

M A King, J V Wells

    Clinical and Experimental Immunology
    |September 1, 1981
    PubMed

    Four patients who had myeloma were examined for monoclonal lymphocytes in the peripheral blood using anti-idiotype antibodies. All the patients had received treatment and two were in apparent complete remission. In no case was there any evidence of peripheral blood mononuclear cells (PBMC) with endogenous surface idiotypic determinants, even in patients with active disease. It was found, however, that substantial cytophilic IgG may remain bound to Fc receptors on PBMC even after extensive incubation and washing at 37 degrees C.

    More Related Videos

    Monitoring PD-1-Blocking Antibodies Bound to T Cells Derived from a Drop of Peripheral Blood06:07

    Monitoring PD-1-Blocking Antibodies Bound to T Cells Derived from a Drop of Peripheral Blood

    Published on: February 5, 2020

    Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis08:44

    Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis

    Published on: September 7, 2022

    Related Concept Videos

    Humoral Immune Responses01:36

    Humoral Immune Responses

    Overview
    Hybridoma Technology01:31

    Hybridoma Technology

    Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
    Hybridoma Selection
    Commonly used fusion techniques — electroporation, polyethylene glycol...
    B Cell Activation and Differentiation01:24

    B Cell Activation and Differentiation

    The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
    When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...