Related Experiment Video
Updated: Aug 10, 2026

Isolation and Culture of Adult Epithelial Stem Cells from Human Skin
Published on: March 31, 2011
Separation of epidermal cells by density centrifugation: a new technique for studies on normal and pathological
Abstract:
Murine keratinocytes, isolated by flotation trypsinization of skin, can be separated into five groups by centrifugation through Percoll, a colloidal silica gradient. Within each group a good correlation was found between density, plating efficiency, morphological appearance, DNA synthesis, and degree of keratinization/cornification. This method can be applied equally well to fetal, newborn, or adult keratinocytes and should be useful in a variety of studies including isolation of subpopulations of pathological cell types, work on chalones and hyperplastic diseases such as psoriasis, and in vitro transformation studies.
Related Concept Videos
Overview Of Cell Separation And Isolation
Subcellular Fractionation
Differential Centrifugation
Differential centrifugation is...
Centrifugation

