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A new chromogenic amylase method compared with two established methods.

J Fenton, R Foery, L Piatt

    Clinical Chemistry
    |April 1, 1982
    PubMed
    Summary

    A new amylase reagent, Pantrak, demonstrates excellent precision and a wide dynamic range. This chromogenic substrate method is reliable for clinical diagnostics, though certain anticoagulants interfere.

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    Area of Science:

    • Clinical Chemistry
    • Biochemical Assays
    • Enzyme Kinetics

    Background:

    • Amylase is a key enzyme in carbohydrate metabolism.
    • Accurate amylase measurement is crucial for diagnosing pancreatic disorders.
    • Existing diagnostic methods require evaluation for improved clinical utility.

    Purpose of the Study:

    • To evaluate the analytical performance of a new chromogenic amylase reagent, Pantrak.
    • To assess its precision, dynamic range, and potential interferences compared to established methods.

    Main Methods:

    • Pantrak utilizes p-nitrophenyl-alpha-maltaosides as chromogenic substrates.
    • Amylase activity is indirectly measured by quantifying p-nitrophenol released via alpha-glucosidase.
    • A series of 100 clinical specimens were analyzed using Pantrak and two reference methods.

    Main Results:

    • Pantrak showed excellent correlation with established amylase testing methodologies.
    • The reagent exhibited good precision, with coefficients of variation (CVs) ranging from 3.0% to 4.5%.
    • Pantrak demonstrated a broad dynamic range up to 800 U/L and was unaffected by common interferents like triglycerides, hemoglobin, and bilirubin.

    Conclusions:

    • The Pantrak amylase reagent possesses desirable analytical characteristics for clinical use.
    • Its ease of use and reliable performance make it a suitable alternative for amylase determination.
    • Caution is advised regarding the use of anticoagulants that chelate divalent cations.

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