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Related Experiment Videos

Electron microscopy in histiocytosis X.

G W Mierau, B E Favara, J M Brenman

    Ultrastructural Pathology
    |April 1, 1982
    PubMed
    Summary

    Electron microscopy reliably detects Langerhans cell granules, aiding histiocytosis X diagnosis. This technique remains sensitive even with suboptimal tissue preservation from paraffin blocks.

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    Area of Science:

    • Pathology
    • Electron Microscopy
    • Cell Biology

    Background:

    • Histiocytosis X diagnosis relies on identifying Langerhans cell granules.
    • Ultrastructural examination is crucial for definitive diagnosis.

    Purpose of the Study:

    • To evaluate the sensitivity of electron microscopy in detecting Langerhans cell granules.
    • To compare diagnostic yield between fresh tissue and paraffin-embedded samples for histiocytosis X.

    Main Methods:

    • Ultrastructural analysis of 24 histiocytosis X cases.
    • Comparison of tissue processed primarily for electron microscopy versus paraffin-embedded tissue.
    • Deparaffinization techniques for analyzing archival material.

    Main Results:

    • Langerhans cell granules were detected in 100% of fresh samples and 67% of paraffin-embedded samples.
    • Detection rate in deparaffinized material was 79% for known positive cases.
    • Incidence of granules decreased from 48% in fresh samples to 14% in paraffin-embedded samples.

    Conclusions:

    • Electron microscopy is a highly sensitive method for diagnosing histiocytosis X.
    • The technique is effective even with suboptimal tissue preservation.
    • Paraffin-embedded tissues can yield diagnostic results with appropriate preparation.

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