Enzyme-linked immunospecific assays for human alphafetoprotein using monoclonal antibodies

Two sandwich-type enzyme-linked immunospecific assays for human alphafetoprotein (AFP) have been developed. In both assays monoclonal antibodies were immobilised by absorption to the wells of microtitre plates and used to trap AFP in the samples offered. Bound AFP was then quantitated by completing the sandwich with peroxidase-conjugated anti AFP, which was either of polyclonal or monoclonal type. The monoclonal to monoclonal sandwich enable AFP measurements to be made in the range 100 to 1000 kU/l. The polyclonal to monoclonal sandwich covered an AFP range of 10 to 1000 kU/l. Both assays were rapid and robust and extremely sparing in the use of antibody.