Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Induction of unique mRNAs by human interferons.

R J Colonno, R H Pang

    The Journal of Biological Chemistry
    |August 25, 1982
    PubMed
    Summary

    Human interferons (IFN-alpha, IFN-beta, IFN-gamma) induce specific messenger RNAs (mRNAs) in fibroblast cells, correlating with antiviral state development. Different IFN types show varied induction levels and mRNA profiles.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Clinical emergence of entecavir-resistant hepatitis B virus requires additional substitutions in virus already resistant to Lamivudine.

    Antimicrobial agents and chemotherapy·2004
    Same author

    Efficacies of entecavir against lamivudine-resistant hepatitis B virus replication and recombinant polymerases in vitro.

    Antimicrobial agents and chemotherapy·2002
    Same author

    Long-term entecavir treatment results in sustained antiviral efficacy and prolonged life span in the woodchuck model of chronic hepatitis infection.

    The Journal of infectious diseases·2001
    Same author

    Antiviral efficacy of lobucavir (BMS-180194), a cyclobutyl-guanosine nucleoside analogue, in the woodchuck (Marmota monax) model of chronic hepatitis B virus (HBV) infection.

    Antiviral research·2001
    Same author

    In vitro resistance profile of the human immunodeficiency virus type 1 protease inhibitor BMS-232632.

    Antimicrobial agents and chemotherapy·2000
    Same author

    BMS-232632, a highly potent human immunodeficiency virus protease inhibitor that can be used in combination with other available antiretroviral agents.

    Antimicrobial agents and chemotherapy·2000

    Area of Science:

    • Molecular Biology
    • Immunology
    • Cell Biology

    Background:

    • Interferons (IFNs) are crucial cytokines involved in innate immunity.
    • IFNs modulate cellular responses, including antiviral defense.
    • Understanding IFN-induced gene expression is key to their therapeutic potential.

    Purpose of the Study:

    • To investigate the mRNA synthesis induced by different types of human interferons (IFN-alpha, IFN-beta, IFN-gamma) in human fibroblast cells.
    • To determine the relationship between induced mRNA accumulation and the development of an antiviral state.
    • To characterize the specific mRNAs and proteins synthesized in response to various IFNs.

    Main Methods:

    • Treatment of human fibroblast cells with human IFN-alpha, IFN-beta, or IFN-gamma.
    • Analysis of newly synthesized mRNAs using a wheat germ cell-free translation system.
    • Quantification of mRNA accumulation and correlation with antiviral state development.
    • Time course studies to observe mRNA appearance and stability.

    Main Results:

    • Human IFNs induced at least four new mRNAs coding for proteins of 56, 57, 62, and 68 kDa.
    • A direct correlation was found between IFN concentration, mRNA accumulation, and antiviral state.
    • IFN-gamma induced the same four mRNAs plus three additional ones, requiring higher concentrations and longer incubation.
    • Mouse interferon induced these mRNAs to a lesser extent in human cells, correlating with limited antiviral response.

    Conclusions:

    • Interferon treatment triggers the synthesis of specific mRNAs that are directly linked to the establishment of an antiviral state.
    • Different types of human interferons exhibit distinct patterns of mRNA induction and antiviral efficacy.
    • The level and type of induced mRNA are critical determinants of the cell's resistance to viral infection.

    Related Experiment Videos