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A self determinant recognizing T cell hybridoma.

D Juy, E Barbier, C Le Guern

    Journal of Immunology (Baltimore, Md. : 1950)
    |September 1, 1982
    PubMed
    Summary
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    A novel T cell hybridoma recognizes mouse and rat red blood cells (RBC). This hybridoma likely identifies shared carbohydrate determinants on murine immunoglobulins and RBCs.

    Area of Science:

    • Immunology
    • Cell Biology
    • Biochemistry

    Background:

    • T cell hybridomas are valuable tools for studying immune responses and cell surface interactions.
    • Understanding antigen recognition by T cells is crucial for immunology research.

    Purpose of the Study:

    • To characterize a newly developed T cell hybridoma, designated 53(113).
    • To investigate the recognition specificities and functional properties of this hybridoma.
    • To explore the molecular basis of antigen recognition by the 53(113) hybridoma.

    Main Methods:

    • Generation of a T cell hybridoma (53(113)) by fusing BALB/c spleen cells with BW 5147 lymphoma cells.
    • Assays for hemagglutination activity using various red blood cells (RBCs).
    • Protein A plaque formation assays with guinea pig complement and mouse serum/immunoglobulins.

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  • Inhibition studies using mouse immunoglobulins and RBC sonicates.

    • Main Results:

    • The 53(113) hybridoma specifically recognizes mouse RBC (MRBC) and rat RBC, but not RBCs from other species.
    • Culture supernatant of 53(113) exhibits hemagglutinating activity against MRBC and rat RBC.
    • 53(113) cells form protein A plaques, indicating interaction with mouse immunoglobulins (Ig) or related structures.
    • Both mouse Ig and MRBC sonicates inhibit hybridoma-RBC rosettes and protein A plaque formation.

    Conclusions:

    • The 53(113) T cell hybridoma recognizes a determinant present on MRBC and mouse Ig.
    • This determinant is likely a carbohydrate structure shared between murine Ig and RBCs.
    • The findings suggest a potential cross-reactivity or shared epitope involved in immune recognition.