Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Angiogenesis and neoplastic progression in vitro.

M Ziche, P M Gullino

    Journal of the National Cancer Institute
    |August 1, 1982
    PubMed
    Summary

    Cell populations gained the ability to form new blood vessels (angiogenesis) during in vitro cultivation before showing signs of neoplastic transformation. This suggests angiogenesis indicates an increased risk of cancer development.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Evidence of β-blockers drug repurposing for the treatment of triple negative breast cancer: A systematic review.

    Neoplasma·2019
    Same author

    ALDH2 Activity Reduces Mitochondrial Oxygen Reserve Capacity in Endothelial Cells and Induces Senescence Properties.

    Oxidative medicine and cellular longevity·2018
    Same author

    Characterization of zofenoprilat as an inducer of functional angiogenesis through increased H2 S availability.

    British journal of pharmacology·2015
    Same author

    EGFR signaling upregulates expression of microsomal prostaglandin E synthase-1 in cancer cells leading to enhanced tumorigenicity.

    Oncogene·2011
    Same author

    A nanoscale fluorocarbon coating on PET surfaces improves the adhesion and growth of cultured coronary endothelial cells.

    Nanotechnology·2011
    Same author

    Angiogenic phenotype induced by basic fibroblast growth factor transfection in brain microvascular endothelial cells.

    International journal of oncology·2011

    Area of Science:

    • Oncology
    • Cell Biology
    • Vascular Biology

    Background:

    • In vivo studies show high-risk cells acquire angiogenesis before neoplastic transformation.
    • Neoplastic transformation is a complex process involving cellular changes leading to cancer.

    Purpose of the Study:

    • To investigate if angiogenic capacity develops during in vitro neoplastic transformation.
    • To determine if angiogenesis precedes recognizable neoplastic transformation in cell cultures.

    Main Methods:

    • Cultivation of four distinct cell populations in vitro: human mammary epithelium (HBL 100), BALB/c fibroblasts, C57BL-MG epithelium, and Syrian golden hamster embryo cells.
    • Monitoring for the appearance of angiogenic capacity during cultivation.
    • Assessing for neoplastic transformation markers.

    Main Results:

    • Angiogenic capacity emerged in all four cell populations during in vitro cultivation.
    • The development of angiogenesis was observed significantly before any signs of neoplastic transformation were detectable.
    • The study utilized multiple cell types to ensure the generalizability of findings.

    Conclusions:

    • Acquisition of angiogenic capacity in vitro can occur independently of, and prior to, recognizable neoplastic transformation.
    • The development of angiogenesis in a cell population suggests an elevated risk for neoplastic transformation.
    • This finding supports the hypothesis linking angiogenesis to early stages of cancer development.

    Related Experiment Videos