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Mast cells and complement activating immune reactions.

H Renner, S Schnitzler

    Agents and Actions. Supplements
    |January 1, 1982
    PubMed
    Summary
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    Isolated rat mast cells do not release histamine when rosetted with complement-coated zymosan. However, this process enhances histamine release induced by ATP, and prednisolone treatment reduces rosette-forming mast cells.

    Area of Science:

    • Immunology
    • Cell Biology
    • Pharmacology

    Background:

    • Mast cells are key immune cells involved in allergic reactions and inflammation.
    • Complement-coated zymosan is a tool used to study mast cell activation.
    • Adenosine triphosphate (ATP) can trigger mast cell degranulation.

    Purpose of the Study:

    • To investigate the effect of complement-coated zymosan on rat mast cell histamine release.
    • To determine if pre-incubation with zymosan affects ATP-induced histamine release.
    • To examine the impact of prednisolone on mast cell rosetting.

    Main Methods:

    • Isolated rat mast cells were incubated with complement-coated zymosan.
    • Histamine release was measured after stimulation with ATP.

    Related Experiment Videos

  • Rats were treated with prednisolone, and mast cell rosetting was assessed.

    • Main Results:

    • Rosetting with complement-coated zymosan alone did not induce histamine release from mast cells.
    • This rosetting procedure potentiated histamine release when subsequently stimulated by ATP.
    • Prednisolone treatment led to a reduction in the number of mast cells capable of forming rosettes.

    Conclusions:

    • Complement-coated zymosan does not directly activate rat mast cells for histamine release.
    • Mast cells pre-sensitized with zymosan exhibit an enhanced response to ATP stimulation.
    • Prednisolone has an inhibitory effect on mast cell rosetting, suggesting modulation of cell surface interactions or mast cell numbers.