Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structure in tRNA data.

H Grosjean, R J Cedergren, W McKay

    Biochimie
    |June 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    This study analyzed 260 transfer RNA (tRNA) sequences across diverse categories. Findings reveal non-random base distributions and selection pressures acting on both variable and invariant tRNA positions during evolution.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Pus1p-dependent tRNA pseudouridinylation becomes essential when tRNA biogenesis is compromised in yeast.

    The Journal of biological chemistry·2001
    Same author

    Identification and characterization of the tRNA:Psi 31-synthase (Pus6p) of Saccharomyces cerevisiae.

    The Journal of biological chemistry·2001
    Same author

    Cloning and characterization of the Schizosaccharomyces pombe tRNA:pseudouridine synthase Pus1p.

    Nucleic acids research·2000
    Same author

    Identification of the Saccharomyces cerevisiae RNA:pseudouridine synthase responsible for formation of psi(2819) in 21S mitochondrial ribosomal RNA.

    Nucleic acids research·2000
    Same author

    RNA:pseudouridine synthetase Pus1 from Saccharomyces cerevisiae: oligomerization property and stoichiometry of the complex with yeast tRNA(Phe).

    Biochimie·1999
    Same author

    Multisite-specific tRNA:m5C-methyltransferase (Trm4) in yeast Saccharomyces cerevisiae: identification of the gene and substrate specificity of the enzyme.

    RNA (New York, N.Y.)·1999
    Same journal

    Differential cardiac microRNA expression in anoxic Trachemys scripta elegans turtles.

    Biochimie·2026
    Same journal

    Renal failure-driven luminal ammonia production impairs gut barrier function in CKD.

    Biochimie·2026
    Same journal

    Conditional Knockout of Indoleamine 2, 3-Dioxygenase-1 in Osteoprogenitor Cells in Mice Results in Sex-dependent Differences in Bone Mass.

    Biochimie·2026
    Same journal

    Sedentariness disrupts, while exercise restores, thermogenic and metabolic plasticity in inguinal adipose tissue of mice.

    Biochimie·2026
    Same journal

    Chrononutrition as a modulator of retinal metabolic resilience: A translational framework linking circadian biology to ocular disease.

    Biochimie·2026
    Same journal

    Heterologous expression, purification, and biophysical characterisation of the cobalt-dependent nitrile hydratase from Rhodococcus rhodochrous ATCC BAA-870.

    Biochimie·2026
    See all related articles

    Area of Science:

    • Molecular Biology
    • Evolutionary Biology
    • Genetics

    Background:

    • Transfer RNA (tRNA) molecules are crucial for protein synthesis.
    • Previous research identified invariant and semi-invariable positions in tRNA sequences.
    • Understanding sequence variations is key to comprehending tRNA function and evolution.

    Purpose of the Study:

    • To analyze sequence variations in a comprehensive set of tRNA molecules.
    • To investigate the distribution of bases and identify selection pressures across different tRNA classes.
    • To explore the evolutionary constraints on tRNA structure and function.

    Main Methods:

    • Comparative sequence analysis of 260 tRNA sequences.
    • Classification of tRNAs into six categories: prokaryotic elongators, eukaryotic elongators, prokaryotic initiators, eukaryotic initiators, lower eukaryotic mitochondrial tRNAs, and archaebacterial tRNAs.

    Related Experiment Videos

  • Statistical analysis of base distribution at conserved and variable positions.

    • Main Results:

    • Identified non-random base distribution across nearly all positions in tRNA sequences.
    • Observed that base distributions are often characteristic of specific tRNA classes.
    • Demonstrated evidence of selection pressures acting on both invariant and variable positions.

    Conclusions:

    • Evolutionary selection pressures influence both conserved and variable sites in tRNA molecules.
    • Functional and structural constraints likely dictate these observed base distributions and selection patterns.
    • The study provides insights into the evolutionary trajectory and functional adaptations of diverse tRNA types.