Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human T cell lines with antigen specificity and helper activity.

A Lanzavecchia, M Ferrarini, F Celada

    European Journal of Immunology
    |June 1, 1982
    PubMed
    Summary

    Human T blasts, stimulated by specific antigens, proliferate in response to those antigens. These T blasts induce B cell proliferation and differentiation, requiring cell-to-cell interactions for activation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    To the editor, response letter.

    Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·2021
    Same author

    Angular distribution of neutron production by proton and carbon-ion therapeutic beams.

    Physics in medicine and biology·2020
    Same author

    Recommendations of the Spanish brachytherapy group (GEB) of Spanish Society of Radiation Oncology (SEOR) and the Spanish Society of Medical Physics (SEFM) for high-dose rate (HDR) non melanoma skin cancer brachytherapy.

    Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·2017
    Same author

    Heterogeneous expression of the collagen receptor DDR1 in chronic lymphocytic leukaemia and correlation with progression.

    Blood cancer journal·2017
    Same author

    Effects of miRNA-15 and miRNA-16 expression replacement in chronic lymphocytic leukemia: implication for therapy.

    Leukemia·2017
    Same author

    lncRNA profiling in early-stage chronic lymphocytic leukemia identifies transcriptional fingerprints with relevance in clinical outcome.

    Blood cancer journal·2016

    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Human T blasts are crucial for adaptive immunity.
    • Understanding T blast function is key to immune response research.

    Purpose of the Study:

    • To investigate the antigen specificity and B cell activation capabilities of human T blasts.
    • To explore the role of cell-cell interactions in T blast-mediated B cell responses.

    Main Methods:

    • Long-term culture of human T blasts from peripheral blood mononuclear cells (PBM).
    • Antigen restimulation and culture with interleukin-2.
    • Assessing T blast proliferation and B cell activation assays.
    • Depletion of E-rosetting cells and use of mitomycin C-treated cells.

    Main Results:

    • Expanded T blasts showed antigen-specific proliferation.
    • T blasts induced polyclonal B cell proliferation and differentiation in autologous PBM.
    • Allogeneic B cell activation required antigen-stimulated T blasts and autologous irradiated mononuclear cells.
    • Efficient B cell activation appeared dependent on cell-to-cell interactions, not solely soluble factors.

    Conclusions:

    • Human T blasts exhibit antigen specificity.
    • T blasts can induce B cell activation, requiring both antigen and autologous accessory cells.
    • Cell-to-cell contact is essential for efficient T blast-mediated B cell activation.

    Related Experiment Videos