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Related Experiment Videos

Automated, quantitative cytopathic effect reduction assay for interferon.

T J Yeh, P T McBride, J C Overall

    Journal of Clinical Microbiology
    |August 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

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    A new, automated assay quickly measures human interferon (IFN) levels. This cytopathic effect reduction assay uses optical density readings for rapid and efficient IFN titer calculation.

    Area of Science:

    • Biotechnology
    • Immunology
    • Assay Development

    Background:

    • Human interferon (IFN) is crucial for antiviral responses.
    • Accurate and efficient IFN quantification is essential for research and therapeutic applications.
    • Existing assays can be time-consuming and labor-intensive.

    Purpose of the Study:

    • To describe a novel, rapid, and automated cytopathic effect reduction assay for human interferon (IFN).
    • To establish an efficient method for determining IFN titers.
    • To optimize the assay for high-throughput analysis.

    Main Methods:

    • Utilized an automated diluter for serial dilutions of IFN in 96-well microtiter plates.
    • Incubated cell monolayers with diluted IFN for a total of 26 hours.

    Related Experiment Videos

  • Quantified IFN titers by measuring optical density of crystal violet-stained monolayers using an automated spectrophotometer.

    • Main Results:

    • The assay provides a rapid method for human IFN quantification.
    • Automated spectrophotometry allowed for quick plate reading (less than 1 minute per plate).
    • The assay is suitable for determining IFN titers efficiently.

    Conclusions:

    • A rapid, automated cytopathic effect reduction assay for human IFN has been successfully developed.
    • This assay offers a significant improvement in speed and efficiency for IFN titer determination.
    • The method is amenable to high-throughput screening and routine laboratory use.