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Related Experiment Videos

Cytoplasmic structure in rapid-frozen axons.

B J Schnapp, T S Reese

    The Journal of Cell Biology
    |September 1, 1982
    PubMed
    Summary

    This study reveals the intricate internal structure of turtle optic nerve axons, detailing distinct domains within the axoplasm and the specific organization of organelles like mitochondria and vesicles. These findings suggest microtubule domains may serve as transport channels for axonal components.

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    OLFACTORY CILIA IN THE FROG.

    The Journal of cell biology·2009

    Area of Science:

    • Neuroscience
    • Cell Biology
    • Biophysics

    Background:

    • The internal organization of axons is crucial for neuronal function and transport.
    • Understanding the axoplasmic structure provides insights into axonal transport mechanisms.

    Purpose of the Study:

    • To elucidate the three-dimensional organization of axoplasm in turtle optic nerves.
    • To characterize the relationship between axonal cytoskeleton and organelles.

    Main Methods:

    • Rapid-freezing of living turtle optic nerves.
    • Fracturing, etching, and rotary shadowing for electron microscopy.
    • Stereo imaging and analysis of fractured axons.

    Main Results:

    • Identified three longitudinal axoplasmic domains: neurofilament bundles, microtubule-rich areas, and a membrane-associated filamentous network.
    • Demonstrated distinct associations of mitochondria and vesicular organelles with cytoskeletal elements.
    • Proposed microtubule domains as channels for organelle transport.

    Conclusions:

    • Axoplasm exhibits a highly organized, domain-specific structure.
    • Microtubule domains are likely key pathways for axonal transport of organelles and other components.
    • The specific arrangement of cytoskeleton and organelles supports models of active axonal transport.

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