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Vascular labelling with monastral blue B.

I Joris, U DeGirolami, K Wortham

    Stain Technology
    |May 1, 1982
    PubMed
    Summary
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    Monastral blue B is a novel, non-toxic substitute for carbon black in vascular labelling experiments. This pigment effectively identifies leaky blood vessels in vivo and is suitable for various microscopic techniques.

    Area of Science:

    • Experimental pathology
    • Vascular biology
    • Histology

    Background:

    • Vascular labelling is a key technique in experimental pathology to identify vessel permeability.
    • Carbon black, a traditional colloidal pigment for vascular labelling, is no longer commercially available.

    Purpose of the Study:

    • To introduce and evaluate Monastral blue B as a viable substitute for carbon black in vascular labelling.

    Main Methods:

    • Intravenous injection of Monastral blue B suspension in vivo.
    • Evaluation of Monastral blue B in whole mounts, paraffin and plastic embedded histologic sections, and electron microscopy.

    Main Results:

    • Monastral blue B provides clear visualization in whole mounts.
    • The pigment is readily visible in both paraffin and plastic embedded histologic sections.

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  • Distinctive appearance observed in electron micrographs.
  • Monastral blue B demonstrated to be non-toxic at the required dosage.

    • Conclusions:

    • Monastral blue B is an effective and safe alternative to carbon black for vascular labelling.
    • This substitute offers versatility across multiple imaging modalities for studying leaky vessels.