Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Characterization of two substance P antisera.

E Brodin, G Nilsson, K Folkers

    Acta Physiologica Scandinavica
    |January 1, 1982
    PubMed
    Summary

    This study tested substance P (SP) antisera for cross-reactivity. Antiserum K 25 binds to biologically active SP fragments, correlating with guinea pig ileum responses.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Simultaneous determination of cholecystokinin, dopamine, glutamate and aspartate in cortex and striatum of the rat using in vivo microdialysis.

    Amino acids·2013
    Same author

    Serum levels of vitamin D are not associated with future risk of venous thromboembolism. The Tromsø Study.

    Thrombosis and haemostasis·2013
    Same author

    Monocyte count and procoagulant functions are associated with risk of venous thromboembolism: the Tromsø study.

    Journal of thrombosis and haemostasis : JTH·2011
    Same author

    The Swedish version of the Haemophilia Activity List.

    Haemophilia : the official journal of the World Federation of Hemophilia·2011
    Same author

    Endogenous thrombin potential (ETP) in plasma from patients with AMI during antithrombotic treatment.

    Thrombosis research·2008
    Same author

    Randomized clinical trial of laparoscopic versus open fundoplication for gastro-oesophageal reflux.

    The British journal of surgery·2004

    Area of Science:

    • Immunology
    • Neuroscience
    • Peptide Chemistry

    Background:

    • Substance P (SP) is a neuropeptide involved in various physiological processes.
    • Accurate detection of SP is crucial for understanding its roles.
    • Existing SP antisera require characterization for specificity.

    Purpose of the Study:

    • To evaluate the cross-reactivity of two substance P (SP) antisera (K 16 and K 25).
    • To determine the binding specificities of these antisera against SP analogues and related peptides.
    • To correlate antiserum binding with the biological activity of SP fragments.

    Main Methods:

    • Radioimmunoassay (RIA) was employed to test the binding of SP antisera.
    • Various SP analogues, partial sequences, and other naturally occurring peptides were used as competitors.
    • Biological activity was assessed using the isolated guinea pig ileum assay.

    Main Results:

    • Both K 16 and K 25 antisera demonstrated C-terminal directionality.
    • Antiserum K 16 showed a pronounced C-terminal preference.
    • Antiserum K 25's binding profile correlated with the known biological activity of SP peptides on guinea pig ileum.

    Conclusions:

    • Antiserum K 25 exhibits specificity for the biologically active region of the substance P molecule.
    • The findings support the use of antiserum K 25 for studying SP's physiological functions.
    • Characterization of antisera is essential for reliable immunological assays in neuroscience research.

    Related Experiment Videos