[Kidney preservation by mechanical perfusion and by hypothermic storage: a comparative study]

Area of Science:

• Nephrology
• Transplantation Medicine
• Surgical Research

Background:

• Hypothermic storage is a common method for preserving organs for transplantation.
• Optimizing preservation time is crucial for successful organ transplantation outcomes.

Purpose of the Study:

• To evaluate the maximum safe preservation time for canine kidneys using hypothermic storage.
• To compare the efficacy of hypothermic storage with hypothermic mechanical perfusion for kidney preservation.

Main Methods:

• Seventy-two canine kidneys were preserved using hypothermia.
• Preservation times ranged from 24 to 72 hours.
• Kidney function was assessed post-transplant using para-aminohippuric acid (PAH) and inulin clearances.

Main Results:

• Twenty-four hours was determined as the maximum safe preservation time for canine kidneys under hypothermic storage.
• Adding furosemide to the flushing solution did not improve immediate kidney function.
• Kidney function after 72 hours of hypothermic mechanical perfusion was significantly better than after 24 hours of static hypothermic storage.

Conclusions:

• Static hypothermic storage of canine kidneys is limited to 24 hours for optimal function.
• Hypothermic mechanical perfusion provides superior organ preservation compared to static storage for extended periods.