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Human lung mast cells: purification and characterization.

E S Schulman, D W MacGlashan, S P Peters

    Journal of Immunology (Baltimore, Md. : 1950)
    |December 1, 1982
    PubMed
    Summary
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    Researchers developed a new method to purify human lung mast cells, enabling detailed studies of inflammatory disorders. This technique significantly improves mast cell purity for advanced research applications.

    Area of Science:

    • Immunology
    • Cell Biology
    • Pharmacology

    Background:

    • Studies on mast cell-mediated inflammatory disorders are limited by the difficulty in purifying human mast cells.
    • Mast cells play a crucial role in allergic reactions and inflammation.

    Purpose of the Study:

    • To develop and validate a reliable method for purifying human lung mast cells to apparent homogeneity.
    • To enable detailed biochemical and pharmacological studies of mast cell function.

    Main Methods:

    • Enzymatic dispersion of lung tissue to create a single-cell suspension.
    • Partial purification using countercurrent centrifugation elutriation (CCE).
    • Affinity column chromatography using anti-penicillin (BPO)-specific IgE for high-purity isolation.

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    Main Results:

    • Enzymatic dispersion yielded initial mast cell purities of 1.2-9.7%.
    • CCE enriched mast cells to up to 70% purity.
    • Affinity chromatography increased purity to 84% (range 65-98%), further enhanced to 100% with short-term culture.

    Conclusions:

    • A robust multi-step protocol effectively purifies human lung mast cells.
    • Purified mast cells remain intact and functional, demonstrating IgE-mediated histamine release.
    • This purification technique facilitates advanced research into mast cell-mediated inflammatory diseases.