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Qualitative differences between dimeric and trimeric stimulation of human basophils.

D W MacGlashan, R P Schleimer, L M Lichtenstein

Journal of Immunology (Baltimore, Md. : 1950)

|January 1, 1983

Summary

Indomethacin enhances histamine release from human basophils stimulated by aggregated IgE, but not by aggregated IgE. This suggests Fc receptor aggregation state impacts cellular enzyme systems differently.

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Differences in functional consequences and signal transduction induced by IL-3, IL-5, and nerve growth factor in human basophils.

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Area of Science:

Immunology
Cellular Biology

Background:

Previous studies showed differing human basophil responses to antigenic vs. anti-IgE antibody stimulation under indomethacin.
This laboratory previously observed distinct basophil responses based on stimulation type.

Purpose of the Study:

To investigate if the type of IgE aggregation on the cell surface explains differential human basophil responses.
To determine the effect of IgE aggregation state on indomethacin-enhanced histamine release.

Main Methods:

Stimulation of human basophils with synthetically cross-linked IgE (trimer and dimer).
Measurement of histamine release.
Assessment of indomethacin's effect on histamine release kinetics.

Main Results:

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Indomethacin enhanced histamine release induced by aggregated IgE (trimer).
Indomethacin did not enhance histamine release induced by dimeric IgE.
The observed differences were not attributable to varying release kinetics.

Conclusions:

The state of Fc receptor aggregation differentially affects cellular enzyme systems.
IgE aggregation state is a critical factor in modulating human basophil activation and histamine release.