[Dihydrotestosterone-binding characteristics in human prostatic adenoma]

Molecular characteristics of androgenic nuclear receptors of the human prostate adenoma have been detected. Stokes radius is 2.3 nm, sedimentation coefficient in the medium with 0.4 M KCl is 3.1 S, mol. w. 31.500 dalton, friction ratio is 1.1. The receptors were mainly localized in the nuclear, rather than in the microsomal and mitochondrial fractions. Comparative studies of dihydrotestosterone (DHT) binding in cytosol of the human prostate adenoma and in the blood serum were performed. The investigation of (NH4)2SO4 salting out, thermal stability, the state during chromatography on DEAE-cellulose and sephadex S-100 has shown that DHT is bound in cytosol preferentially and/or exclusively with protein, identical to testosterone-estradiol-binding blood globulin. It is not recommended that the quantitative control of cytosol DHT receptors be used for diagnostic purposes.