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Related Experiment Videos

Lead staining in the Golgi complex.

J McClintock, M Locke

    Tissue & Cell
    |January 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Lead ions selectively stain specific parts of the vacuolar system, like the Golgi complex, indicating a unique cellular environment. This lead binding differs from acid phosphatase and uranyl staining, highlighting transient cellular processes.

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    Area of Science:

    • Cell Biology
    • Subcellular Ultrastructure
    • Biochemistry

    Background:

    • Lead ions are used in cytochemical staining for phosphatase localization.
    • The vacuolar system, including the Golgi complex (GC) and isolation envelopes (im), has complex functions in cellular processes.
    • Understanding the molecular composition and dynamics of the vacuolar system is crucial for cell biology research.

    Purpose of the Study:

    • To investigate the characteristic binding of lead ions to specific compartments within the vacuolar system.
    • To differentiate lead ion binding patterns from those of acid phosphatase and uranyl staining.
    • To explore the temporal and functional significance of lead ion staining in cellular compartments.

    Main Methods:

    • Fixation of cells (vertebrates and invertebrates) using 2.5% glutaraldehyde.

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  • Staining with lead citrate in acetate or aspartate buffer at pH 5.5-7.2.
  • Comparative analysis of lead staining with acid phosphatase and uranyl staining patterns.

    • Main Results:

    • Lead ions specifically stain parts of the vacuolar system, notably Golgi complex (GC) and isolation envelopes (im), in both vertebrates and invertebrates.
    • Lead staining exhibits a fine particulate stippling within GC cisternal compartments.
    • The distribution of lead stain differs significantly from acid phosphatase and generalized uranyl staining, suggesting it marks a specific cellular environment rather than proteins.

    Conclusions:

    • Lead ion binding characterizes the luminal membrane surfaces of certain vacuolar system compartments.
    • The lead-staining environment within cisternae is dynamic and temporally discontinuous, related to maturation stages.
    • Distinguishing between transient occupants and the compartment itself is essential when characterizing vacuolar system components using lead staining.