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A selective stain for mitotic figures, particularly in the developing brain.

F J Fraser

    Stain Technology
    |July 1, 1982
    PubMed
    Summary
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    A new selective stain effectively differentiates mitotic figures from resting cells in developing tissues. This method uses crystal violet and nuclear fast red for clear, color-coded cell identification in various organs.

    Area of Science:

    • Histology
    • Cell Biology
    • Developmental Biology

    Background:

    • Conventional nuclear stains lack color differentiation between mitotic and resting cells.
    • Existing methods like Hematoxylin and Feulgen are insufficient for selective mitotic figure identification.
    • Autoradiographic counting is often required but not always feasible.

    Purpose of the Study:

    • To develop a selective staining method for mitotic figures.
    • To provide a color-based differentiation between mitotic and resting cells.
    • To facilitate quantitative estimation of cell division in developing tissues.

    Main Methods:

    • Utilized a modified Bouin fixative and 1 N HCl hydrolysis.
    • Employed crystal violet for selective staining of mitotic figures.

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  • Used nuclear fast red as a counterstain for resting cells.

    • Main Results:

    • Achieved intense blue staining of mitotic figures against a red background of resting cells.
    • Successfully applied the method to various species (sheep, guinea pig, rat) and organs.
    • Demonstrated high efficacy in developing rat brain, aiding prenatal development analysis.

    Conclusions:

    • The presented method offers a highly selective and color-based approach for identifying mitotic figures.
    • This technique is valuable for quantitative studies, especially in developing tissues like the brain.
    • The stain is applicable to all organs and simplifies cell recognition in histological analysis.