Related Experiment Videos
Protective effect of L-ascorbic acid in lindane intoxicated rats.
Acta Vitaminologica Et Enzymologica
|January 1, 1982
Summary
Lindane exposure caused growth retardation and liver enzyme dysfunction in male rats. L-ascorbic acid supplementation effectively reversed these toxic effects, restoring normal growth and enzyme activity.
Area of Science:
- Toxicology
- Biochemistry
- Environmental Health
Background:
- Lindane, an organochlorine pesticide, poses risks to animal health.
- Understanding lindane's impact on liver function and metabolic processes is crucial.
- Enzyme activity alterations are key indicators of toxicological effects.
Purpose of the Study:
- To investigate the biochemical effects of lindane administration on male rat liver.
- To evaluate the protective role of L-ascorbic acid against lindane-induced toxicity.
Main Methods:
- Male rats were administered lindane (20 mg/kg/day for 15 days).
- Liver tissue fractions and plasma membranes were analyzed for enzyme activities.
- L-ascorbic acid was administered orally to assess its protective effects.
Main Results:
- Lindane caused significant growth retardation and inhibited succinic dehydrogenase, Mg2+-ATPase, and glucose-6-phosphatase activities.
- Liver plasma membrane enzymes (Mg2+-ATPase, alkaline phosphatase, NADH-dehydrogenase) were inhibited, while 5'-nucleotidase activity was stimulated.
- L-ascorbic acid supplementation normalized growth and reversed enzyme activity alterations caused by lindane.
Conclusions:
- Lindane exposure induces significant hepatic biochemical disturbances and growth impairment in male rats.
- L-ascorbic acid demonstrates a protective effect against lindane toxicity by normalizing enzyme functions and growth.
- This study highlights the potential of vitamin C as an antidote for lindane poisoning.