The effect of in vitro immune complexes on polymorphonuclear leukocyte chemiluminescence

Chemiluminescence (CL) responses were measured following addition of heat-aggregated human gamma globulin (AHG) or bovine serum albumin/antibovine serum albumin (BSA/Anti-BSA) complexes to resting PMNL. These CL responses were two to four fold greater over control values using normal human serum. Furthermore, preincubation of the PMNL suspension with either AHG or BSA/Anti-BSA complexes inhibited the CL response to subsequent stimulation by serum-opsonized zymosan. These results suggest that immune complexes may be detected by PMN-CL.