Supra-ependymal serotoninergic nerves in mammalian brain: morphological, pharmacological and functional studies

Supra-ependymal nerves in mammals (mainly rats) have been shown to contain serotonin (5-hydroxytryptamine, 5-HT) by combined Falck-Hillarp fluorescence histochemistry, ultrastructural monoamine cytochemistry and pharmacology as well as by immunohistochemistry and autoradiography. Supra-ependymal 5-HT cells do not occur. At least in rats, virtually all supra-ependymal nerves contain 5-HT and in our opinion the occasionally described non-5-HT supra-ependymal nerve cells and their processes contribute little to the supra-ependymal nerve plexus (with the possible exception of those cells above the median eminence). The cells of origin of the supra-ependymal 5-HT nerves are situated in raphe nuclei. The axons and terminals (varicosities) contain small and large dense core vesicles in both of which 5-HT is stored. A co-transmitter has not been found among the candidates investigated so far (leu- and met-enkephalin, substance P and gamma-aminobutyric acid (GABA)). The nerves possess uptake mechanisms specific for 5-HT and possibly GABA. Occasionally desmosome-like junctions are observed between 5-HT nerve terminals and ependymal cells but no true synapses. The function of these nerves is not known. They do not appear to regulate ciliary movement, but might influence the shape of ependymal cells.