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Enhancement of human ADCC with interferon
Clinical and Experimental Immunology
|October 1, 1982
Summary
Leukocyte interferon (IF) enhances human antibody-dependent cell-mediated cytotoxicity (ADCC) by augmenting K cells. This suggests human natural killer (NK) and K cells are similar and IF improves ADCC assay sensitivity.
Area of Science:
- Immunology
- Cellular Biology
Background:
- Antibody-dependent cell-mediated cytotoxicity (ADCC) is a crucial immune mechanism.
- Leukocyte interferon (IF) is known to modulate immune cell activity.
Purpose of the Study:
- To investigate the effect of leukocyte interferon (IF) on human antibody-dependent cell-mediated cytotoxicity (ADCC).
- To determine if IF can augment the cytotoxic activity of effector cells in ADCC.
Main Methods:
- Purified leukocyte interferon (IF) was added to ADCC assay cultures.
- Peripheral blood mononuclear cells, specific antiserum, and effector cells were used.
- Cytotoxicity was measured at various effector to target cell ratios and antiserum concentrations.
Main Results:
- IF significantly increased cytolysis in the ADCC assay.
- Augmentation was observed at lower antiserum concentrations.
- Monocyte removal did not affect cytotoxicity, indicating K cell susceptibility to IF.
Conclusions:
- Leukocyte interferon (IF) augments human K cell-mediated ADCC.
- Results support the similarity between human NK and K cells or their regulatory mechanisms.
- IF treatment may enhance the utility of the ADCC assay for detecting immune responses.