The culture of lymphoma cells in diffusion chambers

The leukemia blast cells from peripheral blood of 5 patients with acute leukemia were cultured in diffusion chambers implanted into peritoneal cavity of mice. In two patients with AML an increase of the blast counts was observed, whereas in 3 other cases the leukemic leukocytes number decreased more or less rapidly. The number of CLL lymphocytes cultured in diffusion chambers decreased more slowly in comparison with the control lymphocytes. A small increase in the number of leukemic and normal lymphocytes during culture was noted. The CLL lymphocytes with no acid phosphatase content during the culture in diffusion chambers demonstrated an increase of enzyme activity.