The segmentation of nuclei in circulating neutrophils of laboratory rats

In normal rats, we determined Edwin's (35 +/- 5) and modified (1 +/- 0) segmentation indices, Hynek's numbers (3.2 +/- 0.0) and neutrophil counts with different numbers of nuclear lobes (more than 99 per cent of neutrophils had 2-5 lobes and 0.6 +/- 0.4 per cent of neutrophils had 6 or more nuclear lobes). Neutrophil counts with 6 or more nuclear lobes seem to be sufficient for determination of experimental induction of hypersegmentation in rat neutrophils.