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Related Experiment Videos

CRP-associated determinant expressed by human monocytes.

J F Kearney, G L Gartland, J M Kilpatrick

    Immunological Communications
    |January 1, 1982
    PubMed
    Summary

    Researchers investigated C-reactive protein (CRP) expression on human peripheral blood cells (PBL). They discovered a CRP-like determinant on monocytes, suggesting a novel role for CRP in cellular interactions.

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    Area of Science:

    • Immunology
    • Cell Biology
    • Biochemistry

    Background:

    • C-reactive protein (CRP), an acute phase reactant, exhibits numerous biological activities.
    • CRP is associated with various blood-borne cells, indicating potential cellular interactions.
    • Previous studies described CRP reactions with monoclonal antibodies, including anti-idiotype antibodies.

    Purpose of the Study:

    • To investigate the expression of C-reactive protein (CRP) on human peripheral blood cells (PBL).
    • To identify specific cell populations within PBL that express CRP or CRP-like determinants.
    • To characterize the binding properties of CRP-reactive monoclonal antibodies.

    Main Methods:

    • Utilized two types of CRP-reactive monoclonal antibodies for cell surface analysis.
    • Employed site-specific antibodies, including those reacting dependently and independently of calcium.
    • Analyzed the reactivity of antibodies with human peripheral blood cells (PBL) using flow cytometry or similar techniques.

    Main Results:

    • One site-specific monoclonal antibody showed reactivity with a subpopulation of normal human PBL.
    • This subpopulation was identified as comprising 100% of human monocytes.
    • No reactivity was observed on lymphoid, NK cells, or granulocyte populations.

    Conclusions:

    • A CRP-like determinant is present on the surface of human monocytes.
    • This finding suggests a potential role for CRP in monocyte function and interactions.
    • The determinant is specific to monocytes and not found on other major PBL types.

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