Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bacillus subtilis relG mutant: defect in glucose uptake.

V L Price, J A Gallant

    Journal of Bacteriology
    |January 1, 1983
    PubMed
    Summary

    A Bacillus subtilis mutant (relG) with a relaxed phenotype was studied further. Researchers found the main issue in this mutant is defective glucose uptake, impacting the downshift mechanism.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Vaccines for parasitic diseases.

    Current drug targets. Infectious disorders·2002
    Same author

    Plasmodium falciparum: immunogenicity of alum-adsorbed clinical-grade TBV25-28, a yeast-secreted malaria transmission-blocking vaccine candidate.

    Experimental parasitology·2001
    Same author

    Ribosomes can slide over and beyond "hungry" codons, resuming protein chain elongation many nucleotides downstream.

    Proceedings of the National Academy of Sciences of the United States of America·1998
    Same author

    Expression and purification of correctly processed, active human TACE catalytic domain in Saccharomyces cerevisiae.

    Protein expression and purification·1998
    Same author

    Saccharomyces cerevisiae-secreted fusion proteins Pfs25 and Pfs28 elicit potent Plasmodium falciparum transmission-blocking antibodies in mice.

    Infection and immunity·1998
    Same author

    Inducible expression cassettes in yeast: ADH2.

    Methods in molecular biology (Clifton, N.J.)·1997

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Bacterial Physiology

    Background:

    • The stringent response is a conserved bacterial mechanism regulating gene expression.
    • Mutants exhibiting a relaxed phenotype (rel) are crucial for studying this response.
    • Bacillus subtilis is a model organism for bacterial genetics and physiology.

    Purpose of the Study:

    • To further characterize a previously described Bacillus subtilis mutant (relG) with a relaxed phenotype.
    • To investigate the underlying defect responsible for the relaxed phenotype observed after glucose limitation.
    • To elucidate the role of glucose uptake in the bacterial downshift mechanism.

    Main Methods:

    • Genetic characterization of the relG mutant in Bacillus subtilis.
    • Analysis of bacterial response to nutrient downshift (glucose limitation).
    • Biochemical assays to assess glucose transport and metabolism.

    Main Results:

    • The relG mutant exhibits a relaxed phenotype specifically after glucose limitation.
    • Further analysis revealed that the primary defect in this mutant is impaired glucose uptake.
    • This defect in glucose uptake is directly linked to the observed relaxed phenotype and affects the downshift mechanism.

    Conclusions:

    • The study identifies defective glucose uptake as the primary cause of the relaxed phenotype in the relG mutant of Bacillus subtilis.
    • This finding highlights the critical role of efficient glucose transport in regulating the bacterial stringent response and adaptation to nutrient changes.

    Related Experiment Videos