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Expanded progeny cells of diffuse cytotoxic bone marrow-derived colonies.
Scandinavian Journal of Immunology
|December 1, 1982
Summary
Cytotoxic bone marrow cells lost activity upon expansion but could regain it with interferon or phorbol ester. Interleukin-3 (IL-3) stimulated these cells, not colony-stimulating factor (CSF), indicating a novel growth factor dependency.
Area of Science:
- Hematology
- Immunology
- Cell Biology
Background:
- Bone marrow-derived diffuse colonies exhibit cytotoxic activity.
- These cytotoxic cells are dependent on colony-stimulating factor (CSF) for initial development.
Purpose of the Study:
- To characterize the growth requirements and phenotype of expanded diffuse colony cells (EDCC).
- To investigate the factors influencing the cytotoxic activity and differentiation of EDCC.
Main Methods:
- Expansion of cytotoxic bone marrow colonies in liquid culture with conditioned media.
- Assessment of cytotoxic activity after pretreatment with interferon or phorbol ester.
- Testing proliferation responses to various growth factors, including partially purified interleukin-3 (IL-3).
- Phenotypic analysis using monoclonal antibodies, esterase staining, and ultrastructural examination.
Main Results:
- Expanded diffuse colony cells (EDCC) exhibited myelocytic morphology and lost cytotoxic capacity.
- Cytotoxicity was reinduced by interferon or phorbol ester pretreatment.
- EDCC proliferation was supported by IL-3 but not by traditional CSF sources.
- EDCC expressed Ia antigens and specific esterases but lacked typical myeloid granules.
Conclusions:
- The stimulatory factor for EDCC proliferation is closely related to IL-3, not CSF.
- EDCC represent immature monomyelocytic cells with potential for differentiation.
- Further research is needed to define factors inducing differentiation in these cells.