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Related Experiment Videos

Secretion of human interferons by yeast.

R A Hitzeman, D W Leung, L J Perry

    Science (New York, N.Y.)
    |February 11, 1983
    PubMed
    Summary

    Yeast can produce human interferons (IFNs) by utilizing and processing human signal sequences for secretion. This demonstrates the potential of lower eukaryotes in synthesizing and modifying human therapeutic proteins.

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    Area of Science:

    • Molecular Biology
    • Biotechnology
    • Microbiology

    Background:

    • Human interferons (IFNs) are crucial for antiviral and immune responses.
    • Efficient production of therapeutic proteins like IFNs is a significant challenge in biotechnology.
    • Understanding heterologous protein processing in yeast can advance biopharmaceutical manufacturing.

    Purpose of the Study:

    • To investigate the synthesis and secretion of human interferons (IFN-alpha 1, IFN-alpha 2, IFN-gamma) in the yeast Saccharomyces cerevisiae.
    • To determine if yeast can correctly process human signal sequences for protein secretion.
    • To assess the feasibility of using yeast as a host for producing functional human interferons.

    Main Methods:

    • Construction of plasmids encoding human IFN genes with secretion signals.
    • Expression of these plasmids in Saccharomyces cerevisiae.
    • Isolation and characterization of secreted IFN activity from yeast culture media.
    • Analysis of the amino termini of purified interferons to confirm signal sequence processing.

    Main Results:

    • Successful synthesis and secretion of IFN activity in yeast.
    • A significant portion of secreted IFN-alpha 1 and IFN-alpha 2 exhibited natural mature amino termini.
    • Evidence suggests yeast correctly removed human signal sequences, similar to human cells.
    • Demonstrated functional expression of human interferon genes in a lower eukaryotic system.

    Conclusions:

    • Saccharomyces cerevisiae can effectively utilize and process human signal sequences for protein secretion.
    • Yeast serves as a viable host for the production of biologically active human interferons.
    • This study highlights the potential of yeast in biopharmaceutical production of human proteins.

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