Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Prekeratin biosynthesis in human scalp epidermis.

P T Bladon, P E Bowden, W J Cunliffe

    The Biochemical Journal
    |October 15, 1982
    PubMed
    Summary

    Human scalp prekeratin analysis reveals complex polypeptide mixtures. Phosphorylation explains some charge variations, suggesting post-translational modifications in keratin gene expression.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Response to G. R. Parslow "listen to the message" [biochemistry and molecular biology education 31, 269 (2003)].

    Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology·2011
    Same author

    Obituary: professor peter N. Campbell (1921-2005).

    Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology·2011
    Same author

    Marks' basic medical biochemistry: A clinical approach (second edition).

    Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology·2011
    Same author

    The Strongest boy in the world: How genetic information is reshaping our lives.

    Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology·2011
    Same author

    Hypomagnesaemia due to proton-pump inhibitor therapy: a clinical case series.

    QJM : monthly journal of the Association of Physicians·2010
    Same author

    Epidermolysis bullosa simplex due to KRT5 mutations: mutation-related differences in cellular fragility and the protective effects of trimethylamine N-oxide in cultured primary keratinocytes.

    The British journal of dermatology·2010

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Dermatology

    Background:

    • Human scalp epidermis contains prekeratin polypeptides.
    • Understanding keratin protein structure and modification is crucial for skin biology.

    Purpose of the Study:

    • To analyze the heterogeneity of human scalp epidermal prekeratin polypeptides.
    • To investigate the post-translational modifications of keratin proteins.

    Main Methods:

    • Two-dimensional gel electrophoresis of prekeratin polypeptides.
    • Radiolabeling with amino acids and orthophosphate.
    • In vitro translation of epidermal RNA followed by immunoprecipitation.

    Main Results:

    • Prekeratin bands comprised multiple polypeptides with similar molecular weights but different isoelectric points.
    • Phosphorylation varied among polypeptides, contributing to charge differences.
    • In vitro translation products showed similar SDS-PAGE mobility but different isoelectric properties compared to authentic prekeratins.

    Conclusions:

    • Human scalp prekeratin is a heterogeneous mixture of polypeptides.
    • Post-translational phosphorylation is a key modification affecting keratin charge.
    • Keratin gene products undergo significant post-translational modification before forming mature keratin structures.

    Related Experiment Videos