In vitro analysis of lymphocytes: markers and functions

Current dermatologic literature is becoming replete with articles elucidating current concepts in the pathophysiology of lymphocytic diseases. Much of this knowledge emanates from the increasingly sophisticated and complex methods of identifying lymphocytes. No longer does it suffice to know that B lymphocytes or T lymphocytes are present, but rather the key information involves knowing what subsets of these cells are present and/or their functional status. The reader attempting to assimilate all this information is thus confronted with an overwhelming, as well as frequently changing, array of analytic methods. This paper is presented as a review of thinking, principles, and methods employed in research on lymphocytic disease.