Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Solid-phase radioimmunoassay for substance P.

A Oblin, B Zivkovic

    Journal of Neurochemistry
    |March 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    A new solid-phase immunoassay accurately quantifies substance P in biological samples. This sensitive method provides reliable substance P measurements comparable to traditional radioimmunoassays.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The discriminative stimulus effects of MK-801: Generalization to other N-methyl-D-aspartate receptor antagonists.

    Journal of psychopharmacology (Oxford, England)·2012
    Same author

    SSR591813, a novel selective and partial alpha4beta2 nicotinic receptor agonist with potential as an aid to smoking cessation.

    The Journal of pharmacology and experimental therapeutics·2003
    Same author

    The preclinical pharmacologic profile of tiapride.

    European psychiatry : the journal of the Association of European Psychiatrists·2001
    Same author

    SL651498: an anxioselective compound with functional selectivity for alpha2- and alpha3-containing gamma-aminobutyric acid(A) (GABA(A)) receptors.

    The Journal of pharmacology and experimental therapeutics·2001
    Same author

    Circadian organization and the role of the pineal in birds.

    Microscopy research and technique·2001
    Same author

    Haloperidol-induced catalepsy is absent in dopamine D(2), but maintained in dopamine D(3) receptor knock-out mice.

    European journal of pharmacology·2000
    Same journal

    PBMC circRNA Profiles Distinguish Atypical From Idiopathic Parkinsonism and Track Clinical Severity Across Syndromes.

    Journal of neurochemistry·2026
    Same journal

    From Synapses to Circuits, the Role of KIBRA and the WWC Family in Adaptive Brain Function.

    Journal of neurochemistry·2026
    Same journal

    The Golgi as a Microtubule Organiser in Neurons.

    Journal of neurochemistry·2026
    Same journal

    A PARK9 iPSC-Derived Dopaminergic Neuron Model Enables Drug Screening Targeting Autophagy-Lysosome Pathway Dysfunction in Parkinson's Disease.

    Journal of neurochemistry·2026
    Same journal

    Opposing Estrous Cycle-Dependent Norepinephrine and Dopamine Regulation in Response to Methamphetamine.

    Journal of neurochemistry·2026
    Same journal

    Exercise Snacking in Alzheimer's Disease: A Mechanistic Rationale Based on Repeated Exerkine Signaling.

    Journal of neurochemistry·2026
    See all related articles

    Area of Science:

    • Neuroscience
    • Biochemistry
    • Immunology

    Background:

    • Substance P is a neuropeptide involved in various physiological processes.
    • Accurate quantification of Substance P is crucial for understanding its role in health and disease.
    • Existing methods for Substance P measurement may have limitations in sensitivity or complexity.

    Purpose of the Study:

    • To develop and validate a novel solid-phase immunoassay for the quantification of Substance P.
    • To assess the sensitivity and reliability of the developed assay.
    • To compare the assay's performance with established radioimmunoassay techniques.

    Main Methods:

    • Development of a solid-phase immunoassay utilizing immobilized Substance P and anti-Substance P antibodies.
    • Quantification of the antibody-antigen complex using 125I-protein A.

    Related Experiment Videos

  • Validation of the assay by measuring Substance P concentrations in rat brain regions.
  • Comparison with a standard radioimmunoassay using 125I-tyr-8-Substance P.

    • Main Results:

    • The developed assay demonstrated high sensitivity, detecting as little as 10 pg of Substance P.
    • Measurements of Substance P in rat brain regions showed comparable results to standard radioimmunoassay.
    • The assay proved to be a simple, sensitive, and reliable technique.

    Conclusions:

    • The novel solid-phase immunoassay is a robust method for quantifying Substance P-like immunoreactivity.
    • This assay offers a valuable tool for neuroscientific and biochemical research.
    • The technique's simplicity and sensitivity make it suitable for various biological samples.