Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Determination of procainamide acetylator status.

J J Lima, W J Jusko

    Clinical Pharmacology and Therapeutics
    |January 1, 1978
    PubMed
    Summary

    Renal function variability can affect acetylator status measurements. A new clearance technique using procainamide and N-acetyl-procainamide levels offers the most accurate way to determine individual acetylation rates.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Hyperparasitoids of Xenostigmus bifasciatus (Hymenoptera: Braconidae), parasitoid of Cinara spp. (Hemiptera: Aphididae), in Pinus plantations in Brazil.

    Brazilian journal of biology = Revista brasleira de biologia·2026
    Same author

    X-Ray cardiac angiographic vessel segmentation based on pixel classification using machine learning and region growing.

    Biomedical physics & engineering express·2021
    Same author

    The Duffy antigen receptor for chemokines regulates asthma pathophysiology.

    Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2017
    Same author

    The IGNITE Pharmacogenetics Working Group: An Opportunity for Building Evidence with Pharmacogenetic Implementation in a Real-World Setting.

    Clinical and translational science·2017
    Same author

    Enantiomeric fraction of styrene glycol as a biomarker of occupational risk exposure to styrene.

    Chemosphere·2016
    Same author

    Mathematical modeling of the circadian dynamics of the neuroendocrine-immune network in experimentally induced arthritis.

    American journal of physiology. Endocrinology and metabolism·2016

    Area of Science:

    • Pharmacokinetics
    • Drug Metabolism
    • Biomarker Discovery

    Background:

    • Renal function variability can interfere with phenotyping acetylator status using common drug/metabolite ratios.
    • Accurate assessment of acetylation rate is crucial for personalized drug therapy.

    Purpose of the Study:

    • To compare existing methods and a novel clearance technique for phenotyping acetylation rate.
    • To identify the most reliable method for distinguishing fast and slow acetylators despite renal function variations.

    Main Methods:

    • Utilized computer simulations and patient studies.
    • Compared two established metabolite/drug ratio methods against a proposed clearance technique.
    • Measured steady-state serum concentrations of procainamide and urinary excretion rates of N-acetyl-procainamide.

    Main Results:

    • The proposed clearance technique demonstrated superior discrimination between fast and slow acetylators compared to ratio methods.
    • Apparent acetylation clearance calculation proved most definitive in phenotyping acetylation rate.
    • Renal function variations were effectively accounted for by the clearance method.

    Conclusions:

    • Calculating apparent acetylation clearance from procainamide and N-acetyl-procainamide levels is the definitive method for phenotyping acetylation rate.
    • This clearance technique overcomes limitations of ratio methods affected by renal function.
    • Provides a robust approach for personalized medicine and drug efficacy studies.

    Related Experiment Videos