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Related Experiment Videos

A fixative for use in muscle histochemistry.

B P Croker, E H Bossen, N T Brinn

    The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
    |January 1, 1983
    PubMed
    Summary

    A new fixative solution preserves key muscle enzyme activity, including nicotinamide adenine dinucleotide phosphate (NADH)-tetrazolium reductase and adenosine triphosphatases (ATPases), aiding in the processing and shipping of biopsy specimens.

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    Area of Science:

    • Biochemistry
    • Histology
    • Enzymology

    Background:

    • Muscle histochemistry relies on preserving enzyme activity.
    • Standard fixation methods can compromise enzyme integrity.
    • A need exists for reliable methods to process and ship tissue samples.

    Purpose of the Study:

    • To describe a novel fixative solution for preserving enzyme activity in muscle tissue.
    • To evaluate the efficacy of this fixative for various enzymes.
    • To assess its utility for processing and shipping biopsy specimens.

    Main Methods:

    • Human muscle biopsy and murine muscle, spleen, and kidney tissues were used.
    • Tissues were either fresh frozen or stored in the fixative for up to 1 month before freezing.
    • Cryostat sections were assayed for nicotinamide adenine dinucleotide phosphate (NADH)-tetrazolium reductase, adenosine triphosphatases (ATPases), myoadenylate deaminase (MD), phosphorylase, acid phosphatase, alkaline phosphatase (alk phos), and nonspecific esterase (NSE).

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    Main Results:

    • Fixation preserved NADH, ATPase, and MD activity in muscle tissue.
    • Phosphorylase activity was not preserved.
    • Alkaline phosphatase activity was preserved in murine spleen and kidney, but acid phosphatase and NSE activity were reduced.

    Conclusions:

    • The described fixative is effective for preserving specific enzyme activities in muscle and other tissues.
    • It offers a viable option for processing or shipping human muscle biopsy and experimental tissues under certain conditions.
    • The fixative is not suitable for preserving phosphorylase, acid phosphatase, or nonspecific esterase activity.