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Updated: Aug 11, 2026

Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation
Published on: April 6, 2017
Effect of EDTA and PGE1 on beta-thromboglobulin liberation from platelets
Abstract:
The aim of this study was to evaluate the effect of PGE1 and EDTA on liberation of beta-thromboglobulin (beta TG) from platelets in vitro. Liberation of beta TG was followed in citrated blood at room temperature for 120 minutes after venesection. PGE1 reduced beta TG liberation, and maximal inhibition was attained by concentrations greater than 2 X 10(-6)M. EDTA induced the efflux of beta TG. This EDTA-induced efflux was delayed but not prevented by PGE1 and by citrate; it was not found at 0-4(0)C. Therefore the use of EDTA to prevent beta TG liberation during sampling for in vitro or in vivo studies depends heavily on modifying factors such as PGE1 and low temperature, and on the time taken to process samples. Its effectiveness must be in some doubt where the platelets may be sufficiently stimulated to overcome these modifying influences, or where handling of samples is less than optimal.
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