Related Experiment Video

Updated: Aug 16, 2026

Cell Fractionation of U937 Cells in the Absence of High-speed Centrifugation
06:53

Cell Fractionation of U937 Cells in the Absence of High-speed Centrifugation

Published on: January 18, 2019

Fractionation of chromosomes. II. Use of hydrophobic affinity partition in aqueous two-phase system

D Bandyopadhyay, G Pinaev

    Experimental Cell Research
    |April 1, 1983
    PubMed

    More Related Videos

    Cell Fractionation of U937 Cells by Isopycnic Density Gradient Purification10:19

    Cell Fractionation of U937 Cells by Isopycnic Density Gradient Purification

    Published on: August 12, 2021

    Enriching Subcellular Proteins in Leptospira Using a Triton X-114-Based Fractionation Approach04:25

    Enriching Subcellular Proteins in Leptospira Using a Triton X-114-Based Fractionation Approach

    Published on: August 8, 2025

    Related Concept Videos

    Chromatography: Introduction01:10

    Chromatography: Introduction

    Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
    The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
    Overview Of Cell Separation And Isolation01:20

    Overview Of Cell Separation And Isolation

    Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
    Subcellular Fractionation01:32

    Subcellular Fractionation

    The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
    Differential Centrifugation
    Differential centrifugation is...
    Centrifugation01:05

    Centrifugation

    Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
    Ion-Exchange Chromatography01:09

    Ion-Exchange Chromatography

    Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
    Affinity Chromatography01:03

    Affinity Chromatography

    Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...